The organic compounds and the mobile phase move over the stationary phase due to the capillary action. Separation of black ink by TLC is shown in figure 1. The polar absorbent can be either finely-ground alumina or silica particles. It uses a solid stationary phase made up of a polar absorbent and a liquid mobile phase composed of a single or a mixture of organic solvents. TLC is a chromatography technique responsible for the separation of organic compounds on a mixture based on their relative mobility. Key Terms: Mobile Phase, Mobility, Rf Value, Stationary Phase, Separation, Thin Layer Chromatography (TLC) The organic compounds are separated by manipulating the mobile phase. The absorbent is coated as a thin layer in a glass slide. The stationary (solid) phase is a polar substance in TLC while the mobile (liquid) phase is a single or combination of solvents. Thin layer chromatography (TLC) is a separation method involved in the separation of two or more organic compounds by their distribution between solid and liquid phases. It is calculated in both paper chromatography and TLC. The Rf value is calculated by measuring the relative distance traveled by a particular organic compound with respect to the mobile phase. The Rf value is the retention factor used in the identification of organic compounds in a mixture.